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Estimation of phyllanthin and hypophyllanthin by high performance liquid chromatography in Phyllanthus amarus
Author(s) -
Sharma Anupam,
Singh Ravneet T.,
Handa Sukhdev S.
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040507
Subject(s) - chemistry , phyllanthus , chromatography , lignan , high performance liquid chromatography , traditional medicine , herb , medicinal herbs , stereochemistry , medicine
A reversed phase high performance liquid chromatographic procedure for standardizing a popular hepatoprotective Indian herb “Bhumyamlaki” ( Phyllanthus amarus ) on the basis of two of its bioactive lignans, phyllanthin and hypophyllanthin, has been developed. The method has been found to be sensitive, precise and to record more than 98% recovery of the two lignans. The leaves of P. amarus were found to contain the highest amounts of phyllanthin (0.7% w/w) and hypophyllanthin (0.3% w/w) as compared to the other parts of the plant.