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Determination of cinnamic acid and 4‐coumaric acid in alfalfa ( Medicago sativa L.) cell suspension cultures by gas chromatography
Author(s) -
Orr John D.,
Sumner Lloyd W.,
Edwards Robert,
Dixon Richard A.
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040309
Subject(s) - chemistry , cinnamic acid , chromatography , derivatization , p coumaric acid , medicago sativa , gas chromatography , bstfa , gas chromatography–mass spectrometry , quantitative analysis (chemistry) , detection limit , coumaric acid , mass spectrometry , ferulic acid , biochemistry , botany , biology
A capillary gas chromatographic (GC) method for the assay of cinnamic acid and 4‐coumaric acid in alfalfa ( Medicago sativa L.) cell extracts is described. These compounds were assayed as their trimethylsilyl derivatives; hydrocinnamic acid was used as the internal standard for quantifications. This method was qualitatively validated by GC/mass spectral analysis and quantitatively validated by measuring the extraction recovery from alfalfa cells, the recovery from derivatization, the stability of silylated derivatives, the limits of detection, the linearity of the detector response to increasing amounts of analytes, and the intra‐ and inter‐assay precisions. Cinnamic acid and 4‐coumaric acid were present in alfalfa cell extracts and were resolved from all other components by this method. As little as 4 pmol of each acid could be quantified, and the detector response was linear over a range covering five orders of magnitude. The cellular concentrations of free cinnamic acid and 4‐coumaric acid ranged from ca. 5–100 μmol/kg fresh weight tissue.