Premium
Qualitative and quantitative determination of taxol and related compounds in Taxus cuspidata Sieb et Zucc
Author(s) -
Fang Weishuo,
Wu Yue,
Zhou Jinyun,
Chen Weiming,
Fang Qicheng
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040307
Subject(s) - chemistry , taxane , taxus , bark (sound) , shrub , acetonitrile , paclitaxel , botany , chromatography , traditional medicine , cancer , forestry , medicine , breast cancer , biology , geography
In this paper the determination of taxol and the related compounds 10‐deacetyltaxol, cephalomannine and 10‐deacetylcephalomannine in various parts of Taxus cuspidata Sieb et Zucc. is reported. All four compounds were well separated on a 5 μm Spheri‐5 RP‐18 column with a mobile phase composed of water:acetonitrile:tetra‐hydrofuran (55:35:10). The concentrations of these compounds are quite different for different parts of the plant and are highest in stem bark. Furthermore, the content is related to the age of the plant, the older plants containing larger quantities of taxane diterpenoids. We have also found that the contents of taxol and related compounds in the shrub are usually higher than those in the tree.