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Thin layer chromatography of bilobalide and ginkgolides A, B, C and J on sodium acetate impregnated silica gel
Author(s) -
van Beek Teris A.,
Lelyveld Gerrit P.
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040306
Subject(s) - chemistry , ginkgolides , chromatography , silica gel , thin layer chromatography , sodium , thin layer , organic chemistry , layer (electronics) , ginkgo biloba , medicine , pharmacology
A normal phase thin layer chromatographic procedure for the separation and detection of bilobalide and ginkgolides A, B, C and J has been developed. The method is based on the impregnation of commercially available silica gel F254 plates with sodium acetate. Silica gel thus impregnated gives a good separation of all major terpene trilactones in ginkgo leaves (0.06 ⩽ Δ R f 0.17) with the solvent systems ethyl acetate: hexane (9:1) (non‐saturated chamber) or 100% methyl acetate (saturated chamber). The sodium acetate has two functions: it greatly improves the separation of the ginkgolide pairs G‐A/G‐B and G‐J/G‐C, and it enhances the sensitivity of the acetic anhydride spray reagent. Quantities of less than 1 μg of all four ginkgolides and bilobalide can be detected.