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The determination of triptolide from Tripterygium hypoglaucum and T. wilfordii by high resolution gas chromatography
Author(s) -
Liang Zhang,
ZhengXing Zhang,
LongSheng Sheng,
DengKui An
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040305
Subject(s) - triptolide , chemistry , tripterygium wilfordii , chromatography , terpenoid , chloroform , gas chromatography , trimethylsilyl , resolution (logic) , tripterygium , stereochemistry , organic chemistry , glycoside , medicine , apoptosis , biochemistry , alternative medicine , pathology , artificial intelligence , computer science
A method has been developed to determine the content of the bioactive diterpenoid triptolide in specimens of Tripterygium hypoglaucum and T. wilfordii obtained from 21 different districts of China. Triptolide was extracted from powdered plant material with chloroform and, following removal of alkaloids, esterified with CH 2 N 2 and silylated with N ‐(trimethylsilyl)imidazole. Samples were analysed quantitatively by high resolution gas chromatography with n ‐hexatriacontane as internal standard. The minimum level of detection of triptolide was 25 ng and mean recoveries of 96% (relative standard deviation 8.7%) were recorded for extracts containing 25–75 μg/mL of the diterpenoid.