A rapid high performance liquid chromatographic method for the separation of the alkaloid precursor L‐tyrosine and six tetrahydroisoquinoline alkaloids of  Papaver somniferum | Zendy

Kraml Marlene M. | Zendy; Dicosmo Frank | Zendy

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A rapid high performance liquid chromatographic method for the separation of the alkaloid precursor L‐tyrosine and six tetrahydroisoquinoline alkaloids of Papaver somniferum

Author(s) -

Kraml Marlene M.,

Dicosmo Frank

Publication year - 1993

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.2800040304

Subject(s) - chemistry , thebaine , chromatography , papaver , sanguinarine , noscapine , benzylisoquinoline , high performance liquid chromatography , alkaloid , papaverine , triethylamine , tetrahydroisoquinoline , morphine , codeine , organic chemistry , pharmacology , medicine , ecology , biology , enzyme , biosynthesis

A high performance liquid chromatographic (HPLC) method was developed for the qualitative and quantitative determination of L‐tyrosine and six common tetrahydroisoquinoline alkaloids (papaverine, noscapine, sanguinarine, morphine, codeine and thebaine) of Papaver somniferum. The reversed phase HPLC method yields baseline separation of the alkaloids in 20 min and is achieved using a simple H 2 O: MeOH linear gradient. Silanol effects commonly associated with the separation of such strongly basic compounds were minimized by the addition of the amine modifier, triethylamine, to the mobile phase.

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