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Microassay to measure phytoalexin response in protoplasts of bean leaves
Author(s) -
Soriano Eva,
García Ernesto
Publication year - 1993
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800040206
Subject(s) - phytoalexin , isoflavonoid , elicitor , chemistry , absorbance , protoplast , chromatography , botany , biochemistry , flavonoid , biology , resveratrol , gene , antioxidant
Phytoalexins are secondary metabolites that in legumes are typically isoflavonoid derivatives. Techniques for their measurement very often use determination of the UV absorbance of ethanol extracts to avoid time‐consuming extractions and the use of expensive solvents. However, other phenolic compounds also give UV absorbances interfering with quantitative determinations. A microassay is proposed to extract and measure bean phytoalexins from intact, viable, bean leaf protoplasts. Protoplasts as a tool for measuring phytoalexin elicitor stimulation is discussed.