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The determination of catecholamines in Crataegus oxyacantha L. Flowers and leaves by high pressure liquid chromatography‐electrochemical detection
Author(s) -
Saija A.,
Germano M. P.,
Marzullo D.,
Imperatore C.,
Campo S.,
De Pasquale R.
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030604
Subject(s) - chemistry , chromatography , chloroform , petroleum ether , monoamine neurotransmitter , high performance liquid chromatography , extraction (chemistry) , crataegus , dopamine , botany , biochemistry , receptor , neuroscience , biology , serotonin
A combined extraction system for the selective and quantitative determination of the monoamines noradrenaline, adrenaline, dopamine and L‐DOPA from the flowers and leaves of Crataegus oxyacantha L. has been developed. The method is based on a preliminary isolation of monoamines by successive petroleum ether and chloroform clean‐up, absorption on alumina and passage through Sep‐Pak C18 cartridges, followed by measurement of the catecholamines by high pressure liquid chromatography, with electrochemical detection, on a reversed phase column. The described method appears sufficiently simple, sensitive, precise and accurate for routine use and holds great promise for pharmacognostic studies.