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A comparative study of different amberlite XAD resins in flavonoid analysis
Author(s) -
TomásBarberán Francisco A.,
Blázquez M. Amparo,
GarciaViguera Cristina,
Ferreres Federico,
TomásLorente Francisco
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030407
Subject(s) - amberlite , chemistry , chromatography , fractionation , flavonoid , polystyrene , adsorption , quercitrin , methanol , desorption , organic chemistry , polymer , antioxidant
Abstract A comparative study of the recovery of flavonoids (both aglycones and glycosides) by different Amberlite XAD resins has been achieved. No significant differences in the percentages of recovery were found with the chemical nature and physical characteristics of the different resins. A flavonoid recovery higher than 90% is possible in a number of cases. The possibility of fractionating the adsorbed flavonoids by desorption with methanol: water mixtures has also been studied. The polystyrene resins (XAD‐2, XAD‐4 and XAD‐16) were found to be more useful for fractionation purposes than the polyacrylic resins (XAD‐7, XAD‐8). The results suggest that Amberlite XAD‐2 is the most suitable for the recovery and fractionation of flavonoids from plant extracts and foods rich in sugars and other polar compounds such as honey and fruit jams.