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Separation, identification and quantification of monomers from cutin polymers by high performance liquid chromatography and evaporative light scattering detection
Author(s) -
Gérard Hervé C.,
Osman Stanley F.,
Fett William F.,
Moreau Robert A.
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030310
Subject(s) - chemistry , cutin , chromatography , monomer , citrus paradisi , cucurbita pepo , gas chromatography , botany , organic chemistry , polymer , rutaceae , biochemistry , biology
A silicic acid high performance liquid chromatographic method which utilizes an evaporative light scattering detector has been developed for the separation of cutin monomers. This binary gradient system was successfully applied to separate underivatized monomers of several fruit cutins including those from apple cvs. Golden Delicious and Red Delicious ( Malus pumila ), tomato ( Lycopersicon esculentum ), grapefruit ( Citrus paradisi ) green pepper ( Capsidum annum ) and pumpkin ( Cucurbita pepo ). All cutin monomers, from the least polar, monohydroxy saturated and unsaturated fatty acids, to the most polar, trihydroxy saturated and unsaturated fatty acids, were separated. Monomer identity was confirmed by gas chromatography‐mass spectromety of collected peaks. The gradient was also able to separate positional isomers of dihydroxy fatty acids, a separation not yet achieved by gas chromatography. The relationship between detector response and the mass of five common cutin monomers was obtained.