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Analysis of carotenoids by high performance liquid chromatography and diode‐array detection
Author(s) -
Bramley Peter M.
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030302
Subject(s) - chemistry , chromatography detector , chromatography , detector , ultraviolet , carotenoid , high performance liquid chromatography , diode , analytical chemistry (journal) , optoelectronics , optics , materials science , biochemistry , physics
The use of diode‐array detectors with high performance liquid chromatography enables the separation and analysis of carotenoids from biological samples to be achieved in a significantly shorter period than when conventional ultraviolet/visible detectors are used. This relates to the ability of diode‐array detectors to provide full spectral analysis of the compounds during chromatography. The features of diode‐array detectors, including their advantages and drawbacks, are discussed, particularly with respect to the identification and quantitation of carotenoids.