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Preparation of radioactively labelled isodityrosine
Author(s) -
Miller Janice G.,
Fry Stephen C.
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030204
Subject(s) - chemistry , molar , tyrosine , chromatography , molar ratio , catalysis , nuclear chemistry , radiochemistry , organic chemistry , biochemistry , medicine , dentistry
Oxidation of L‐[U‐ 14 C]tyrosine with 2.7 molar equivalents of alkaline hexacyanoferrate(III) yielded at least 12 chromatographically mobile oxidation products and a large amount of immobile material. Use of 0.23 molar equivalents of hexacyanoferrate(III) yielded [ 14 C]dityrosine (∼1.3% of added [ 14 C]tyrosine) and [ 14 C]isodityrosine (∼0.6%). A chromatographic method is described for the isolation of these two products from the mixture. A method is also described for the prepration of [ 3 H]isodityrosine from non‐radioactive isodityrosine by catalytic exchange with 3 H 2 . The [ 3 H]isodityrosine formed was essentially stable in 6M HCI at 110°C, indicating that the tritiation occurred at the benzylic groups.