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A kinetic method for assay of plant peroxidase and catalase activities by chemiluminescence
Author(s) -
Frébort Ivo,
Haviger Antonín,
Jílek Milan
Publication year - 1992
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800030203
Subject(s) - chemistry , chemiluminescence , peroxidase , catalase , guaiacol , horseradish peroxidase , radical , chromatography , enzyme , biochemistry
A chemiluminescent method for the simultaneous assay of peroxidase and catalase activities and chemical traps and quenchers of free radicals in natural samples, based on the additions of peroxidase standards, is presented. The method was standardized with horseradish peroxidase and beef liver catalase. The theoretical basis, mathematical treatment and computer processing of the method are described. The technique was used to analyse 25 samples of various common fruits and vegetables. The levels of peroxidase activity thus determined were compared with those obtained using the guaiacol method and were found to be in good agreement. The relationship between the shape of the chemiluminescent curves and the proportions of peroxidase and catalase activities and traps and quenchers are discussed.