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Comparison of radioimmunoassay and capillary gas chromatography in the analysis of l ‐hyoscyamine from plant material
Author(s) -
Martinsen Aarne,
Naaranlahti Toivo,
Turkia Marja Liisa,
Lehtola Timo,
Oksanen Jari,
Ylinen Markku
Publication year - 1991
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800020404
Subject(s) - hyoscyamine , chemistry , tropane , chromatography , gas chromatography , radioimmunoassay , capillary gas chromatography , alkaloid , extraction (chemistry) , solanaceae , stereochemistry , biochemistry , gene
Abstract l ‐Hyoscyamine in the roots of Atropu belladonna L. was assayed by capillary gas chromatography (CCC). When the extraction and analysis were evaluated using a radioimmunoassay (RIA) established for l ‐hyoscyamine, the two methods were found to be positively and significantly correlated. Important characteristics of CCC and RIA methods in tropane alkaloid analysis of plant material are discussed. A prominent cross‐reaction for apoatropine, a constituent of A. belladonna roots and leaves, was found in this study. This cross‐reaction has not been investigated in other studies of hyoscyamine RIA.