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Analysis of cytochalasins in cultures of Ascochyta spp. and in infected plants by high performance liquid and thin layer chromatography
Author(s) -
Capasso Renato,
Evidente Antonio,
Randazzo Giacomino,
Vurro Maurizio,
Bottalico Antonio
Publication year - 1991
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800020210
Subject(s) - ascochyta , thin layer chromatography , chemistry , liquid culture , cytochalasin , botany , chromatography , ornamental plant , thin layer , layer (electronics) , biology , biochemistry , organic chemistry , blight , cytoskeleton , cell
High performance liquid chromatography and high performance thin layer chromatography were used in combination to check the occurrence of the bioactive cytochalasins A, B, C, D, E, H and J in cultures of some phytopathogenic species of Ascochyta , isolated from infected plants. Cytochalasins A and B were readily detected and estimated in the culture filtrates of Ascochyta heteromorpha Sch. and Sacc., the causal agent of foliar disease of oleander ( Nerium oleander L.). The two compounds were also detected in oleander leaves naturally infected by A. heteromorpha suggesting that these compounds play a role in the disease of the ornamental plant. None of the above mentioned cytochalasins were detected in the culture filtrates of the other fungi tested.