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High performance liquid chromatographic determination of alkaloids from Vinca minor L.
Author(s) -
Proksa B.,
Grossmann E.
Publication year - 1991
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800020206
Subject(s) - chemistry , alkaloid , vinca , chromatography , high performance liquid chromatography , methanol , organic chemistry , botany , biology
Fourteen alkaloids from Vinca minor L. were separated and their concentration determined by high performance liquid chromatography using Separon SGX C18 with a mobile phase of methanol:water (0.02 mol/L NH 4 Cl, pH 8.2 adjusted with Et 3 N). The major alkaloids of the leaves were vincamine (0.13%), 1,2‐dehydroaspidospermidine (0.06%) and vincaminoreine (0.028%). The dominant alkaloid of the root was 1,2‐dehydroaspidospermidine (0.28%). The bisindole alkaloid vincarubine was found only in the leaves (0.007%).