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Malondialdehyde titration with thiobarbiturate in plant extracts: Avoidance of pigment interference
Author(s) -
Hagege Daniel,
Nouvelot André,
Boucaud Jean,
Gaspar Thomas
Publication year - 1990
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800010208
Subject(s) - chemistry , malondialdehyde , absorbance , titration , acetone , trichloroacetic acid , chromatography , autoxidation , pigment , nuclear chemistry , biochemistry , antioxidant , organic chemistry
Application of the thiobarbiturate acid (TBA) reaction to pigmented plant tissues (sugarbeet green leaves and calli) leads to an overestimation of malondialdehyde (MDA) concentration. A method of MDA estimation for pigmented plant tissues has been developed, using the trichloroacetic acid precipitated protein pellet, with acetone depigmentation, before TBA reaction. Absorbance is measured between 532 and 590 nm.