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HPLC Analysis of the main alkaloids from Eschscholtzia Californica cham
Author(s) -
Guédon D.,
Cappelaere N.,
Simanek V.
Publication year - 1990
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800010206
Subject(s) - sanguinarine , chemistry , protopine , chelerythrine , chromatography , high performance liquid chromatography , alkaloid , quantitative analysis (chemistry) , stereochemistry , organic chemistry , protein kinase c , enzyme
A rapid prechromatographic sample preparation and a reversed phase high performance liquid chromatographic (RP‐HPLC) ion pair method have been developed for the qualitative and quantitative analysis of the main alkaloids of Eschscholtzia californica , namely allocryptopine, californidine, chelerythrine, eschscholtzine, N ‐methyllaurotetanine, protopine and sanguinarine. The identity and purity of the component HPLC‐peaks were established by photodiode array detection and by comparison with standards. The distribution of alkaloids in the organs of the plant was examined: allocryptopine is the main constituent in roots, whilst californidine is the major alkaloid in all aerial organs except flowers where the main compound is eschscholtzine. HPLC fingerprint chromatograms of extracts of E. californica from different commercial sources were found to be similar.