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The estimation of macromolecular synthesis in plant cell cultures
Author(s) -
Mahady Gail B.,
Beecher Christopher W. W.
Publication year - 1990
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800010202
Subject(s) - chemistry , macromolecule , suspension culture , plant cell , dna , rna , biochemistry , uridine , extraction (chemistry) , nucleic acid , chromatography , cell culture , computational biology , gene , biology , genetics
A simple and relatively rapid technique is described which can be used to determine accurately the overall pattern of macromolecule biosynthesis in plant cells. Sanguinaria canadensis L. (Papaveraceae ) suspension cells were used as the model system. The patterns of DNA, RNA and protein synthesis were determined through cellular incorporation of [methyl‐ 3 H]‐thymidine, [5‐ 3 H]‐uridine and [4,5‐ 3 H]‐leucine, respectively. The method takes advantage of a one‐step filtering procedure, which simplifies the extraction methods normally used. The results are described and possible applications of this procedure in plant‐cell growth studies and biotechnology are discussed.