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Quantitative glucosinolate analysis in Brassica seeds by X‐ray fluorescence spectroscopy
Author(s) -
Schnug E.,
Haneklaus S.
Publication year - 1990
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800010108
Subject(s) - glucosinolate , chemistry , rapeseed , brassica , fluorescence spectroscopy , spectroscopy , analytical chemistry (journal) , fluorescence , sulfur , calibration , chromatography , botany , food science , mathematics , organic chemistry , statistics , optics , physics , quantum mechanics , biology
X‐ray fluorescence spectroscopy (X‐RF) is an indirect method which is suitable for the quantification of the total glucosinolate content of Brassica seeds. The X‐RF method is based on two major principles: the close relationship between total sulphur and total glucosinolate content in rapeseed and the distinctive applicabilities of X‐ray fluorescence spectroscopy for total sulphur determination in organic matter. The whole preparation procedure consists of only three steps and needs less than five minutes per sample. A special advantage of the method is the availability of a centralized calibration system which ensures the comparability of data derived from different laboratories.