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Comparison of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium by simultaneous determination of multi‐components with single reference standard method and chemometric analysis
Author(s) -
Guo Long,
Zhang Dan,
Xue Zijing,
Jiao Qian,
Liu Aipeng,
Zheng Yuguang,
Liu EHu,
Duan Li
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2786
Subject(s) - folium of descartes , traditional medicine , chemistry , phytochemical , chemometrics , chromatography , medicine , high performance liquid chromatography
Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, two morphologically similar herbal medicines derived from Artemisia genus. Although the two Artemisia herbs have been used as medicines for a long time in China, the study of their phytochemical and bioactive composition is limited. Objective To comprehensively compare and evaluate the composition of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, and find the chemical makers for quality evaluation of the two Artemisia herbal medicines. Methodology Eight compounds including six phenolic acids and two flavonoids were assayed by a single reference standard for simultaneous determination of multi‐components method using 3‐caffeoylquinic acid as the reference standard. The quantitative data were further analysed by chemometric approaches to compare and distinguish the two herbal medicines. Results The credibility and feasibility of the single reference standard for simultaneous determination of the multi‐components method were carefully validated. The validated method was applied to analyse 16 batches of Artemisiae argyi Folium and 10 batches of Artemisiae lavandulaefoliae Folium samples. The quantitative results showed that 3,5‐di‐ O ‐caffeoylquinic acid was the most abundant constituent, and the contents of flavonoids were notably different between the two herbal medicines. The chemometric analysis results indicated the two flavonoids, jaceosidin and eupatilin could be used as chemical markers for quality evaluation of the two herbal medicines. Conclusion The single reference standard for simultaneous determination of the multi‐components method coupled with chemometrics analysis could be a well‐acceptable strategy to compare and evaluate the quality of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium.