Premium
Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry
Author(s) -
Chen Guilin,
Huang Bill X.,
Guo Mingquan
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2769
Subject(s) - chemistry , ultrafiltration (renal) , mass spectrometry , small molecule , chromatography , high throughput screening , liquid chromatography–mass spectrometry , human health , biological activity , drug discovery , computational biology , biochemical engineering , biochemistry , medicine , environmental health , engineering , in vitro , biology
Medicinal plants have played an important role in maintaining human health for thousands of years. However, the interactions between the active components in medicinal plants and some certain biological targets during a disease are still unclear in most cases. Objective To conduct the high‐throughput screening for small active molecules that can interact with biological targets, which is of great theoretical significance and practical value. Methodology The ultrafiltration mass spectrometry (UF‐LC/MS) is a powerful bio‐analytical method by combining affinity ultrafiltration and liquid chromatography‐mass spectrometry (LC/MS), which could rapidly screen and identify small active molecules that bind to biological targets of interest at the same time. Compared with other analytical methods, affinity UF‐LC/MS has the characteristics of fast, sensitive and high throughput, and is especially suitable for the complicated extracts of medicinal plants. Results In this review, the basic principle, characteristics and some most recent challenges in UF‐LC/MS have been demonstrated. Meanwhile, the progress and applications of affinity UF‐LC/MS in the discovery of the active components from natural medicinal plants and the interactions between small molecules and biological target proteins are also briefly summarised. In addition, the future directions for UF‐LC/MS are also prospected. Conclusion Affinity UF‐LC/MS is a powerful tool in studies on the interactions between small active molecules and biological protein targets, especially in the high‐throughput screening of active components from the natural medicinal plants.