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Metabolomic profiling and biological investigation of the marine sponge‐derived bacterium Rhodococcus sp. UA13
Author(s) -
Elsayed Yasmin,
Refaat John,
Abdelmohsen Usama Ramadan,
Othman Eman Maher,
Stopper Helga,
Fouad Mostafa Ahmed
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2765
Subject(s) - chemistry , chromatography , metabolomics , ethyl acetate , sponge , fractionation , enterococcus faecalis , biochemistry , biology , botany , escherichia coli , gene
Marine sponge‐associated actinomycetes are potent sources of bioactive natural products of pharmaceutical significance. They also contributed to the discovery of several clinically relevant antimicrobials. Objective To apply the non‐targeted metabolomics approach in chemical profiling of the sponge‐derived bacterium Rhodococcus sp. UA13, formerly recovered from the Red Sea sponge Callyspongia aff. Implexa , along with testing for the anti‐infective potential of its different fractions. Methodology Metabolomic analysis of the crude extract was carried out using liquid chromatography with high resolution electrospray ionisation mass spectrometry (LC‐HR‐ESI‐MS) for dereplication purposes. Besides, the three major fractions (ethyl acetate, methanol, and n ‐butanol) obtained by chromatographic fractionation of the crude extract were evaluated for their anti‐infective properties. Results A variety of metabolites, mostly peptides, were characterised herein for the first time from the genus Rhodococcus . Among the tested samples, the n ‐butanol fraction showed potent inhibitory activities against Staphylococcus aureus , Candida albicans , and Trypanosoma brucei brucei with IC 50 values of 9.3, 6.7, and 8.7 μg/mL, respectively, whereas only the ethyl acetate fraction was active against Chlamydia trachomatis (IC 50 = 18.9 μg/mL). In contrast, both fractions did not exert anti‐infective actions against Enterococcus faecalis and Leishmania major , whereas the methanol fraction was totally inactive against all the tested organisms. Conclusion This study showed the helpfulness of the established procedure in metabolic profiling of marine actinomycetes using liquid chromatography mass spectrometry (LC–MS) data, which aids in reducing the complex isolation steps during their chemical characterisation. The anti‐infective spectrum of their metabolites is also interestingly relevant to future drug development.