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Qualitative and quantitative analysis of lipo‐alkaloids and fatty acids in Aconitum carmichaelii using LC–MS and GC–MS
Author(s) -
Liang Ying,
Yan GuanYu,
Wu JianLin,
Zong Xingxing,
Liu Zhongqiu,
Zhou Hua,
Liu Liang,
Li Na
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2760
Subject(s) - chemistry , aconitum , chromatography , qualitative analysis , gas chromatography–mass spectrometry , aconitine , alkaloid , organic chemistry , mass spectrometry , qualitative research , social science , sociology
Lipo‐alkaloid (LA) is a kind of C 19 ‐norditerpenoid alkaloid in Aconitum species, which usually contains an aconitane skeleton and one or two fatty acid residues. Objective To qualify and quantify the fatty acids and lipo‐alkaloids in Aconitum carmichaelii . Methodology An ultra‐high performance liquid chromatography‐triple quadrupole‐mass spectrometry (UHPLC‐QQQ‐MS) method was established to quantify LAs, while the free fatty acids were identified by gas chromatography–mass spectrometry (GC–MS) and ultra‐high performance liquid chromatography‐quadrupole‐time‐of‐flight‐mass spectrometry (UHPLC‐Q‐TOF‐MS). Results Six major LAs ( 1–6 ) containing linoleic, palmitic, and oleic acid residues as side chains were quantified. Eighteen fatty acids were determined by GC–MS, and 15 were detected as the side chains of LAs. The LAs containing these 15 fatty acid residues accounted for about a third of the total identified LAs. Moreover, the contents of linoleic, palmitic, and oleic acids were highest. In addition, 12 oxygenated fatty acids were also identified by UHPLC‐Q‐TOF‐MS for the first time. Conclusion The positive correlation between free fatty acids and LAs in A. carmichaelii indicated that the types and contents of LAs were influenced by free fatty acids.