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Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry
Author(s) -
Han Shengli,
Lv Yanni,
Wei Fen,
Fu Jia,
Hu Qi,
Wang Sicen
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2756
Subject(s) - chemistry , chromatography , mass spectrometry , liquid chromatography–mass spectrometry
Abstract Introduction Cell membrane chromatography (CMC), as a highly selective type of affinity chromatography, has been demonstrated as an effective method to screen bioactive components acting on specific receptor from a complicated biological system. Objective To review the recent research progress and the technical applications of these analytical methods using CMC combined with gas chromatography‐mass spectrometry, (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS). Methodology In this review, we briefly introduce the CMC offline GC/MS, CMC online GC/MS, CMC offline LC/MS, and CMC online LC/MS system. And the practical application of these technologies is also enumerated. Then the future of these technologies and research methods were discussed. Results Many bioactive components interacting with specific receptors have been screened and identified in traditional Chinese medicines. Conclusion CMC technique has been combined with GC/MS and HPLC/MS and these combined systems have been successfully used to screen bioactive components acting on specific receptors from a complicated biological system.