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Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC‐MS/MS
Author(s) -
Siegle Lydia,
Pietsch Jörg
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2748
Subject(s) - taxus , aril , chemistry , chromatography , extraction (chemistry) , taxane , high performance liquid chromatography , botany , food science , medicine , cancer , breast cancer , biology
Taxus baccata L . is an evergreen conifer whose plant parts are cardiotoxic. Only the red arils of the berries are described as non‐toxic and taxane‐free. Objective Extraction and HPLC‐MS/MS methods were developed for the investigation of the Taxus compounds 3,5‐dimethoxyphenol, 10‐deacetylbaccatin III, baccatin III, cephalomannine, taxol A and taxinine M in the red arils of the yew berries. Methodology A liquid–liquid extraction method for the red arils of the fruits from three yews were developed. An accurate (ESI+) HPLC‐MS/MS method was performed for the simultaneous detection and determination of the target compounds in multiple reaction monitoring (MRM) mode. Results All Taxus agents obtained were detected in the red arils. Highest concentrations were determined for baccatin III and 10‐deacetylbaccatin III. Conclusion The developed quantitative method is reliable and selective and was successfully applied for quantification of selected Taxus ingredients in red arils of Taxus baccata . It was disproved that the red arils of the berries do not contain the selected Taxus compounds.