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Quantification of Incensole in Three Boswellia Species by NIR Spectroscopy Coupled with PLSR and Cross‐Validation by HPLC
Author(s) -
AlShidhani Sulaiman,
Rehman Najeeb Ur,
Mabood Fazal,
AlBroumi Muhammed,
Hussain Hidayat,
Hussain Javid,
Csuk Rene,
AlHarrasi Ahmed
Publication year - 2018
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2743
Subject(s) - chemistry , partial least squares regression , chromatography , high performance liquid chromatography , near infrared spectroscopy , boswellia serrata , chemometrics , analytical chemistry (journal) , medicine , statistics , physics , mathematics , alternative medicine , quantum mechanics , pathology
Incensole can be considered as a biomarker for Boswellia species which is a diterpene that has received remarkable pharmacological interest recently due to its potent anti‐inflammatory and anti‐depressant activity. Objective Near‐infrared (NIR) spectroscopy coupled with PLSR (partial least squares regression) as a robust, rapid and alternative method was used to quantify the content of incensole in three species namely B. papyrifera , B. sacra and B. serrata and cross‐validated by high‐performance liquid chromatography (HPLC). Materials and Methods NIR spectrophotometer was used for the quantification of incensole standards and Boswellia species in absorption mode in the wavelength range between 700 and 2500 nm. A PLSR model was built from the obtained spectral data using 70% of the incensole working standard solutions (training set), ranging from 0.5 to 100 ppm. The PLSR model obtained has a R 2 value of 98% with a correlationship of 0.99 and a good prediction with root mean square error for prediction (RMSEP) value of 3.2%. Results The results indicated that the methanol (MeOH) extract of B. papyrifera resin has the highest concentration of incensole (18.4%) followed by n ‐hexane (13.5%) and ethyl acetate (3.6%) while trace amounts was detected in the fractions of B. sacra and no incensole was detected in the fractions of B. serrata . Conclusion The findings are in total agreement with the HPLC analysis suggesting that NIR spectroscopy coupled with PLSR is a robust, rapid and non‐destructive alternate method for the quantification of incensole in B. papyrifera . Copyright © 2018 John Wiley & Sons, Ltd.