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Mass Spectrometry Imaging of low Molecular Weight Compounds in Garlic ( Allium sativum L.) with Gold Nanoparticle Enhanced Target
Author(s) -
Misiorek Maria,
Sekuła Justyna,
Ruman Tomasz
Publication year - 2017
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2696
Subject(s) - allium sativum , chemistry , mass spectrometry imaging , allium , alliin , mass spectrometry , colloidal gold , nanoparticle , food science , chromatography , nanotechnology , botany , materials science , biology , allicin
Garlic ( Allium sativum ) is the subject of many studies due to its numerous beneficial properties. Although compounds of garlic have been studied by various analytical methods, their tissue distributions are still unclear. Mass spectrometry imaging (MSI) appears to be a very powerful tool for the identification of the localisation of compounds within a garlic clove. Objective Visualisation of the spatial distribution of garlic low‐molecular weight compounds with nanoparticle‐based MSI. Methodology Compounds occurring on the cross‐section of sprouted garlic has been transferred to gold‐nanoparticle enhanced target (AuNPET) by imprinting. The imprint was then subjected to MSI analysis. Results The results suggest that low molecular weight compounds, such as amino acids, dipeptides, fatty acids, organosulphur and organoselenium compounds are distributed within the garlic clove in a characteristic manner. It can be connected with their biological functions and metabolic properties in the plant. Conclusion New methodology for the visualisation of low molecular weight compounds allowed a correlation to be made between their spatial distribution within a sprouted garlic clove and their biological function. Copyright © 2017 John Wiley & Sons, Ltd.