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Qualitative Analysis of Polyphenols in Macroporous Resin Pretreated Pomegranate Husk Extract by HPLC‐QTOF‐MS
Author(s) -
Abdulla Rahima,
Mansur Sanawar,
Lai Haizhong,
Ubul Ablikim,
Sun Guangying,
Huang Guozheng,
Aisa Haji Akber
Publication year - 2017
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2695
Subject(s) - chemistry , husk , polyphenol , chromatography , rutin , high performance liquid chromatography , kaempferol , glucoside , punica , quercetin , traditional medicine , antioxidant , biochemistry , botany , medicine , alternative medicine , pathology , biology
Pomegranate ( Punica granatum L.) husk is a traditional herbal medicine abundant in phenolic compounds and plays some roles in the treatment of oxidative stress, bacterial and viral infection, diabetes mellitus, and acute and chronic inflammation. Objective Identification and determination of polyphenols in macroporous resin pretreated pomegranate husk extract by high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry (HPLC‐QTOF‐MS). Methods The total polyphenols of pomegranate husk were prepared by ethanol extraction followed by pretreatment with HPD‐300 macroporous resin. The polyphenolic compounds were qualitatively analysed by HPLC‐QTOF‐MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. Results A total of 50 polyphenols were detected in the extract of pomegranate husk, including 35 hydrolysable tannins and 15 flavonoids with distinct retention time, fragmentation behaviours and characteristics, and the accurate mass‐to‐charge ratios at low, moderate and high CE values. Of these, we identified nine compounds for the first time in the pomegranate husk, including hexahydroxydiphenoyl‐valoneoyl‐glucoside (HHDP‐valoneyl‐glucoside), galloyl‐ O ‐punicalin, rutin, hyperoside, quercimeritrin, kaempferol‐7‐ O ‐rhahmano‐glucoside, luteolin‐3′‐ O ‐arabinoside, luteolin‐3′‐ O ‐glucoside, and luteolin‐4′‐ O ‐glucoside. To validate the specificity and accuracy of mass spectrometry in the detection of polyphenols, as compared to the fragmentation pathways of granatin B in detail, including the HHDP‐valoneyl‐ glucoside was first identified from pomegranate husk. Conclusion The study provides evidence for the quality control and development of novel drugs based on polyphenols from the pomegranate husk. Copyright © 2017 John Wiley & Sons, Ltd.