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Untargeted Metabolomic Analysis of Capsicum spp. by GC–MS
Author(s) -
Aranha Bianca Camargo,
Hoffmann Jessica Fernanda,
Barbieri Rosa Lia,
Rombaldi Cesar Valmor,
Chaves Fábio Clasen
Publication year - 2017
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2692
Subject(s) - chemistry , metabolomics , germplasm , pepper , gas chromatography–mass spectrometry , chromatography , sugar , mass spectrometry , gas chromatography , food science , botany , biology
In order to conserve the biodiversity of Capsicum species and find genotypes with potential to be utilised commercially, Embrapa Clima Temperado maintains an active germplasm collection (AGC) that requires characterisation, enabling genotype selection and support for breeding programmes. Objective The objective of this study was to characterise pepper accessions from the Embrapa Clima Temperado AGC and differentiate species based on their metabolic profile using an untargeted metabolomics approach. Material and Methods Cold (˗20°C) methanol extraction residue of freeze‐dried fruit samples was partitioned into water/methanol (A) and chloroform (B) fractions. The polar fraction (A) was derivatised and both fractions (A and B) were analysed by gas chromatography coupled to mass spectrometry (GC–MS). Data from each fraction was analysed using a multivariate principal component analysis (PCA) with XCMS software. Results Amino acids, sugars, organic acids, capsaicinoids, and hydrocarbons were identified. Outlying accessions including P116 ( C. chinense ), P46, and P76 ( C. annuum ) were observed in a PCA plot mainly due to their high sucrose and fructose contents. PCA also indicated a separation of P221 ( C. annuum ) and P200 ( C. chinense ), because of their high dihydrocapsaicin content. Conclusions Although the metabolic profiling did not allow for grouping by species, it permitted the simultaneous identification and quantification of several compounds complementing and expanding the metabolic database of the studied Capsicum spp. in the AGC. Copyright © 2017 John Wiley & Sons, Ltd.