Premium
Immunoaffinity Knockout of Saponin Glycosides from Asparagus racemosus to Assess Anti‐lipid Peroxidation
Author(s) -
Onlom Churanya,
Phrompittayarat Watoo,
Putalun Waraporn,
Waranuch Neti,
Ingkaninan Kornkanok
Publication year - 2017
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2678
Subject(s) - chemistry , saponin , asparagus racemosus , glycoside , lipid peroxidation , chromatography , triterpene , biochemistry , tbars , traditional medicine , antioxidant , stereochemistry , medicinal plants , medicine , alternative medicine , pathology
Asparagus racemosus Willd (Asparagaceae family), known as Shatavari, is important in Ayurveda and traditional Thai medicines. The saponin glycosides, shatavarin I and IV are major constituents in its roots and may be responsible for their actions including protection against lipid peroxidation and carcinogenesis. Objective To develop an immunoaffinity column for isolating compounds with structures related to shatavarin IV from crude extracts of A. racemosus root. Methodology The monoclonal antibody recognising shatavarin IV (mAbShavIV) was coupled to an Affi‐Gel Hz gel to isolate compounds with structures related to shatavarin IV from the other components of crude extracts of A. racemosus root. The saponin glycosides in each fraction were analysed by mAbShavIV ELISA and LC–MS/MS. Results The pooled wash‐through fractions contained 3% of loaded mAbShavIV reactive saponin glycosides, while eluted fractions released ~ 90% of shatavarin saponin glycosides in a single step. Using thiobarbiturate (TBARs) to measure lipid‐peroxidation, the extract, and the pooled wash‐through fractions showed moderate protection against Cu + ‐induced oxidation of human low density lipoprotein (LDL) (IC 50 11.3 ± 1.4 and 12.6 ± 0.9 μg/mL, respectively). In contrast, the saponin glycosides eluted from the mAbShavIV‐column had weaker protectant (IC 50 29.7 ± 1.8 μg/mL) suggesting that A. racemosus shatavarins do not inhibit carcinogenesis through preventing lipid peroxidation. Conclusion The strategy described here demonstrates its utility for isolating a group of related compounds from the rest of the extract with selectivity and recovery rate. Pharmacological efficacy and synergistic effects of the components obtained can be further investigated. Copyright © 2017 John Wiley & Sons, Ltd.