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Capillary Electrophoresis as Tool for Diastereomeric Separation in a Trichilia catigua Fraction
Author(s) -
Sereia Ana Luiza,
Longhini Renata,
Lopes Gisely Cristiny,
Mello João Carlos Palazzo
Publication year - 2016
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2653
Subject(s) - chemistry , capillary electrophoresis , chromatography , diastereomer , cyclodextrin , high performance liquid chromatography , polyphenol , catechin , stereochemistry , organic chemistry , antioxidant
The tree Trichilia catigua , popularly known as “catuaba”, shows several biological activities and has emerged as a potential source of new drugs. Considering that more than 10 species are known under the same popular name, regulatory agencies require more rigorous quality control of this medicinal plant. Objective To develop and validate a methodology using capillary electrophoresis (CE) with ultraviolet (UV) detection for analysing polyphenols in the ethyl‐acetate fraction (EAF) of Trichilia catigua . Methodology Different electrophoretic conditions (such as wavelength of UV detection, voltage, buffer concentration and pH, cyclodextrin type and concentration) were investigated. After optimisation, borate buffer 80 mmol/L at pH 8.80 with 2‐hydroxypropyl‐β‐cyclodextrin 10 mmol/L was selected as background electrolyte. A voltage reduction was used to improve the separation of a diastereomeric pair of cinchonains. Results The method proved to be simple, sensitive, accurate, linear, precise and reproducible. For the first time in natural products analysis, a voltage reduction and hydroxypropyl‐ β ‐cyclodextrin were used to improve the separation of diastereomeric pairs. Until now, this is the only described methodology able to separate catechin, epicatechin, cinchonains Ia, Ib, IIa, and IIb from Trichilia catigua samples on the same run in less than 12 min. When compared to the high performance liquid chromatography with photo‐diode array detection (HPLC‐PDA) method previously developed by our research group, the CE method was more efficient, faster, less expensive and less polluting. Conclusion Our results demonstrate that this method could be employed in a quality‐control laboratory for the quantification of polyphenols in EAF of Trichilia catigua . Copyright © 2016 John Wiley & Sons, Ltd.