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The establishment of efficient bioconversion, extraction, and isolation processes for the production of phyllodulcin, a potential high intensity sweetener, from sweet hydrangea leaves ( Hydrangea macrophylla Thunbergii )
Author(s) -
Jung ChangHwan,
Kim Yang,
Kim MinSoo,
Lee Suyong,
Yoo SangHo
Publication year - 2016
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2609
Subject(s) - bioconversion , chemistry , extraction (chemistry) , methanol , sonication , chromatography , sucrose , solvent , ethanol , botany , fermentation , food science , biochemistry , organic chemistry , biology
Abstract Introduction Hydrangea leaf tea has been traditionally consumed in the far‐east Asian countries and is favoured for its distinct minty‐sweet taste. Phyllodulcin is identified as a key sweet‐tasting compound; it is 400–800 times sweeter than sucrose. However, its extraction has not been well‐documented. In an effort to optimise phyllodulcin production, pretreatment processes to accumulate phyllodulcin as a final metabolite in leaf tissue were studied, and an efficient process was established for the extraction and purification of phyllodulcin.Methods Phyllodulcin was structurally identified using an LC/MS system. Hydrangea leaves were processed by either hand rolling or mechanical blending, by exposing them at different drying temperatures (25 and 70 °C ), and even by inducing bioconversion in leaf tissue. The leaf powder was extracted with various solvents (methanol, ethanol, and water) by soaking at 25 °C for 12 h, ultrasonication at 35 °C for 1 h or accelerated solvent extraction (ASE). Extracts were purified with ion exchange resins and purified using preparative HPLC.Results Traditional hand rolling and drying at 70 °C significantly increased phyllodulcin accumulation in the leaves. Meanwhile, more phyllodulcin was obtained from the leaves blended mechanically or converted enzymatically compared to traditionally processed ones ( P < 0.05). Methanol and ethanol were superior to water as extraction media, and the greatest phyllodulcin yields obtained by ASE, soaking and ultrasonication were 21.28, 21.20 and 19.33 mg/g, respectively, when methanol was used. Highly pure phyllodulcin powder was obtained with a yield of 2.12%.Conclusions This promising result would be beneficial to the industrial utilisation of phyllodulcin as a potential high‐intensity sweetener . Copyright © 2016 John Wiley & Sons, Ltd.