Premium
Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma Brucei
Author(s) -
Omar Ruwida M. K.,
Igoli John,
Gray Alexander I.,
Ebiloma Godwin Unekwuojo,
Clements Carol,
Fearnley James,
Edrada Ebel Ru Angeli,
Zhang Tong,
De Koning Harry P.,
Watson David G.
Publication year - 2015
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2605
Subject(s) - propolis , trypanosoma brucei , chemistry , pinocembrin , trypanosoma brucei rhodesiense , liquiritigenin , biochemistry , food science , flavonoid , antioxidant , medicine , alternative medicine , pathology , gene
A previous study showed the unique character of Nigerian red propolis from Rivers State, Nigeria (RSN), with regards to chemical composition and activity against Trypanosoma brucei in comparison with other African propolis. Objective To carry out fractionation and biological testing of Nigerian propolis in order to isolate compounds with anti‐trypanosomal activity. To compare the composition of the RSN propolis with the composition of Brazilian red propolis. Methodology Profiling was carried out using HPLC‐UV‐ELSD and HPLC‐Orbitrap‐FTMS on extracts of two samples collected from RSN with data extraction using MZmine software. Isolation was carried out by normal phase and reversed phase MPLC. Elucidation of the compounds with a purity > 95% was performed by 1D/2D NMR HRMS and HRLC‐MS n . Results Ten phenolic compounds were isolated or in the case of liquiritigenin partially purified. Data for nine of these correlated with literature reports of known compounds i.e. one isoflavanone, calycosin (1); two flavanones, liquiritigenin (2) and pinocembrin (5); an isoflavan, vestitol (3); a pterocarpan, medicarpin (4); two prenylflavanones, 8‐prenylnaringenin (7) and 6‐prenylnaringenin (8); and two geranyl flavonoids, propolin D (9) and macarangin (10). The tenth was elucidated as a previously undescribed dihydrobenzofuran (6). The isolated compounds were tested against Trypanosoma brucei and displayed moderate to high activity. Some of the compounds tested had similar activity against wild type T. brucei and two strains displaying pentamidine resistance. Conclusion Nigerian propolis from RSN has some similarities with Brazilian red propolis. The propolis displayed anti‐trypanosomal activity at a potentially useful level. Copyright © 2015 John Wiley & Sons, Ltd.