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Application of diffusion‐edited and solvent suppression 1 H‐NMR to the direct analysis of markers in valerian‐hop liquid herbal products
Author(s) -
Prieto Jose M.,
MellinasGomez Maria,
Zloh Mire
Publication year - 2016
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2603
Subject(s) - chemistry , valerian , humulus lupulus , solvent , hop (telecommunications) , nuclear magnetic resonance spectroscopy , chromatography , qualitative analysis , organic chemistry , traditional medicine , food science , pepper , computer science , qualitative research , social science , sociology , medicine , computer network
Abstract Introduction – The rising trend to consume herbal products for the treatment and/or prevention of minor ailments together with their chemical and pharmacological complexity means there is an urgent need to develop new approaches to their quality and stability. Objectives – This work looks at the application of one‐dimensional diffusion‐edited 1 H‐NMR spectroscopy (1D DOSY) and 1 H‐NMR with suppression of the ethanol and water signals to the characterisation of quality and stability markers in multi‐component herbal medicines/food supplements. Material and Methods – The experiments were performed with commercial tinctures of Valeriana officinalis L. (valerian), expired and non‐expired, as well as its combination with Hummulus lupulus L. (hops), which is one of the most popular blends of relaxant herbs. These techniques did not require purification or evaporation of components for the qualitative analysis of the mixture, but only the addition of D 2 O and TSP. Results – The best diagnostic signals were found at δ 7 ppm (H‐11, valerenic acid), δ 4.2 ppm (H‐1, hydroxyvalerenic acid) and δ 1.5‐1.8 ppm (methyl groups in prenylated moieties, α‐acids/prenylated flavones). Conclusion – This work concludes on the potential value of 1D DOSY 1 H‐NMR to provide additional assurance of quality in complex natural mixtures. Copyright © 2016 John Wiley & Sons, Ltd.