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Investigation of the Role of the Calvin Cycle and C1 Metabolism during HCHO Metabolism in Gaseous HCHO‐Treated Petunia under Light and Dark Conditions Using 13 C‐NMR
Author(s) -
Sun Huiqun,
Zhang Wei,
Tang Lijuan,
Han Shuang,
Wang Xinjia,
Zhou Shengen,
Li Kunzhi,
Chen Limei
Publication year - 2015
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2556
Subject(s) - chemistry , petunia , metabolism , detoxification (alternative medicine) , formaldehyde , biochemistry , gene , medicine , alternative medicine , pathology
It has been shown that formaldehyde (HCHO) absorbed by plants can be assimilated through the Calvin cycle or C1 metabolism. Our previous study indicated that Petunia hybrida could effectively eliminate HCHO from HCHO‐polluted air. Objective To understand the roles of C1 metabolism and the Calvin cycle during HCHO metabolism and detoxification in petunia plants treated with gaseous H 13 CHO under light and dark conditions. Methods Aseptically grown petunia plants were treated with gaseous H 13 CHO under dark and light conditions. The metabolites generated from HCHO detoxification in petunia were investigated using 13 C‐NMR. Results [2‐ 13 C]glycine (Gly) was generated via C1 metabolism and [U‐ 13 C]glucose (Gluc) was produced through the Calvin cycle simultaneously in petunia treated with low‐level gaseous H 13 CHO under light conditions. Generation of [2‐ 13 C]Gly decreased whereas [U‐ 13 C]Gluc and [U‐ 13 C]fructose (Fruc) production increased greatly under high‐level gaseous H 13 CHO stress in the light. In contrast, [U‐ 13 C]Gluc and [U‐ 13 C] Fruc production decreased greatly and [2‐ 13 C]Gly generation increased significantly under low‐level and high‐level gaseous H 13 CHO stress in the dark. Conclusion C1 metabolism and the Calvin cycle contributed differently to HCHO metabolism and detoxification in gaseous H 13 CHO‐treated petunia plants. As the level of gaseous HCHO increased, the role of C1 metabolism decreased and the role of the Calvin cycle increased under light conditions. However, opposite changes were observed in petunia plants under dark conditions. Copyright © 2015 John Wiley & Sons, Ltd.