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Revealing Metabolomic Variations in Cortex Moutan from Different Root Parts using HPLC–MS method
Author(s) -
Xiao Chaoni,
Wu Man,
Chen Yongyong,
Zhang Yajun,
Zhao Xinfeng,
Zheng Xiaohui
Publication year - 2014
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2539
Subject(s) - paeonol , paeonia suffruticosa , chemistry , gallic acid , metabolomics , metabolite , primary metabolite , cortex (anatomy) , kaempferol , chromatography , botany , biochemistry , flavonoid , biology , medicine , alternative medicine , pathology , neuroscience , antioxidant
The distribution of metabolites in the different root parts of Cortex Moutan (the root bark of Paeonia suffruticosa Andrews) is not well understood, therefore, scientific evidence is not available for quality assessment of Cortex Moutan. Objective To reveal metabolomic variations in Cortex Moutan in order to gain deeper insights to enable quality control. Methods Metabolomic variations in the different root parts of Cortex Moutan were characterised using high‐performance liquid chromatography combined with mass spectrometry (HPLC–MS) and multivariate data analysis. The discriminating metabolites in different root parts were evaluated by the one‐way analysis of variance and a fold change parameter. Results The metabolite profiles of Cortex Moutan were largely dominated by five primary and 41 secondary metabolites . Higher levels of malic acid, gallic acid and mudanoside‐B were mainly observed in the second lateral roots, whereas dihydroxyacetophenone, benzoyloxypaeoniflorin, suffruticoside‐A, kaempferol dihexoside, mudanpioside E and mudanpioside J accumulated in the first lateral and axial roots. The highest contents of paeonol, galloyloxypaeoniflorin and procyanidin B were detected in the axial roots. Accordingly, metabolite compositions of Cortex Moutan were found to vary among different root parts. Conclusion The axial roots have higher quality than the lateral roots in Cortex Moutan due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading Cortex Moutan on the general market. Copyright © 2014 John Wiley & Sons, Ltd.