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Simultaneous Characterisation of Fifty Coumarins from the Roots of Angelica dahurica by Off‐line Two‐dimensional High‐performance Liquid Chromatography Coupled with Electrospray Ionisation Tandem Mass Spectrometry
Author(s) -
Li Bin,
Zhang Xu,
Wang Juan,
Zhang Le,
Gao Bowen,
Shi Shepo,
Wang Xiaohui,
Li Jun,
Tu Pengfei
Publication year - 2014
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2496
Subject(s) - chemistry , chromatography , electrospray , electrospray ionization , imperatorin , tandem mass spectrometry , mass spectrometry , high performance liquid chromatography , coumarin , organic chemistry
The root of Angelica dahurica is a traditional Chinese medicine that used for the treatment of headache, toothache, abscess, furunculosis and acne. Coumarins were the major bioactive constituents of A. dahurica , hence it is worthwhile developing a method to simultaneously characterise them, especially those in trace amounts. Objective To develop an efficient method for the simultaneous characterisation of coumarins in A. dahurica . Methods A method using off‐line two‐dimensional high‐performance liquid chromatography coupled with electrospray tandem mass spectrometry (off‐line 2D‐HPLC–ESI/MS n ) was developed. Results In total 50 coumarins, including 32 linear furanocoumarins, 16 bifuranocoumarins and two non‐furanocoumarins, were identified from the roots of A. dahurica . The possible MS fragmentations of these coumarins are also proposed. Conclusion The method described here allows rapid and convenient identification of the coumarins in A. dahurica , and may be applied to other herbal medicines containing linear furanocoumarins. Copyright © 2014 John Wiley & Sons, Ltd.