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C ‐glucosidic Ellagitannins from Lythri herba ( European Pharmacopoeia ): Chromatographic Profile and Structure Determination
Author(s) -
Piwowarski Jakub P.,
Kiss Anna K.
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2415
Subject(s) - chemistry , chromatography , phytochemical , tannin , electrospray , high performance liquid chromatography , electrospray ionization , mass spectrometry , biochemistry , food science
Lythri herba, a pharmacopoeial plant material ( European Pharmacopoea ), is obtained from flowering parts of purple loosestrife ( Lythrum salicaria L.). Although extracts from this plant material have been proven to possess some interesting biological activities and its pharmacopoeial standardisation is based on total tannin content determination, the phytochemical characterisation of this main group of compounds has not yet been fully conducted. Objective To isolate ellagitannins from Lythri herba, determine their structures and develop chromatographic methods for their qualitative analysis. Results Five C ‐glucosidic ellagitannins – monomeric‐ vescalagin and castalagin together with new dimeric structures – salicarinins A–C, composed of vescalagin and stachyurin, vescalagin and casuarinin, castalagin and casuarinin units connected via formation of valoneoyl group, were isolated using column chromatography and preparative HPLC. Structures were determined according to 1 H and 13 C‐NMR (one‐ and two‐dimensional), electrospray ionisation–time of flight (ESI–TOF), electrospray ionisation‐ion trap (ESI‐MS n ) and circular dichroism (CD) spectra, together with acidic hydrolysis products analysis. HPTLC on RP‐18 modified plates and HPLC–DAD–MS n on RP‐18 column methods were developed for separation of the five main ellagitannins. Copyright © 2012 John Wiley & Sons, Ltd.