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Rapid Identification of Unstable Acyl Glucoside Flavonoids of Oxytropis racemosa Turcz by High‐performance Liquid Chromatography–Diode Array Detection–Electrospray Ionisation/Multi‐stage Mass Spectrometry
Author(s) -
Song Shuang,
Zheng XiuPing,
Liu WeiDong,
Du RuiFang,
Feng ZiMing,
Zhang PeiCheng,
Bi LiFu
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2395
Subject(s) - chemistry , chromatography , high performance liquid chromatography , mass spectrometry , electrospray ionization , phytochemical , electrospray , glycoside , stereochemistry , biochemistry
Oxytropis racemosa Turcz is an important minority medicine that is used mainly to improve children's indigestion, especially in inner Mongolia and Tibet. Previous studies indicated that the characteristic constituents of this plant are acylated flavonoids. Objective Rapidly identify the characteristic chemical constituents of O. racemosa by high‐performance liquid chromatography–diode array detection–electrospray ionisation/multi‐stage mass spectrometry (HPLC–DAD–ESI/MS n ) and suggest a useful method to control the quality of this medicinal plant. Methods In the HPLC fingerprint, 32 flavonoids were tentatively identified by a detailed analysis of their mass spectra, UV spectra and retention times. Furthermore, 13 flavonoids were confirmed by comparison with previously isolated compounds obtained from O. racemosa . Results In total, 32 flavonoids, including 13 flavonoids with 3‐hydroxy‐3‐methylglutaric acid (HMG) moieties and four flavonoids with 3‐malonyl moieties, were identified in the extract of O. racemosa . Among the compounds identified, 10 were characterised as new compounds for their particular acylated sugar moieties. Conclusions The method described is effective for obtaining a comprehensive phytochemical profile of plants containing unstable acylated flavonoids. The method is also useful for constructing the chromatographic fingerprint of the minority medicine – O. racemosa Turcz for quality control. Copyright © 2012 John Wiley & Sons, Ltd.