Premium
Quantitation of α‐Galactosides in Rehmannia glutinosa by Hydrophilic Interaction Chromatography–Evaporative Light Scattering Detector
Author(s) -
Kim Tae Bum,
Kim Seung Hyun,
Sung Sang Hyun
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2362
Subject(s) - chemistry , stachyose , chromatography , raffinose , rehmannia glutinosa , food science , medicine , alternative medicine , traditional chinese medicine , pathology , sucrose
The use of raffinose family oligosaccharides (RFOs) to control several diseases and stimulate immune systems has been studied. Rehmannia glutinosa is reported to contain RFOs; however, there are no reports on their content level in R. glutinosa . Furthermore, it is difficult to analyse RFOs using common reverse‐phase chromatographic techniques due to their high polarity. Objective To develop a rapid and reliable chromatographic method for the simultaneous quantitation of multiple RFOs in R. glutinosa . Methodology Chromatographic separation was performed by using hydrophilic interaction liquid chromatography coupled with an evaporative light scattering detector. For efficient ultrasound‐assisted extraction of oligosaccharides, a central composite design was used. Results Response surface analysis estimated the highest‐obtained yields of raffinose, stachyose and verbascose to be 58.31, 408.75 and 26.59 mg, respectively, from 1 g of R. glutinosa , while under optimal conditions, experimental yields per gram were 56.79, 391.42 and 27.69 mg, respectively. Conclusion A rapid and reliable chromatographic method to simultaneously quantitate multiple RFOs in R. glutinosa was developed. Three RFOs in R. glutinosa were separated within 10 min using isocratic conditions. Quantitative analysis of RFOs showed that R. glutinosa may be a rich source of α‐galacto‐oligosaccharides, especially stachyose. Copyright © 2012 John Wiley & Sons, Ltd.