Premium
Determination of Macrocyclic Ellagitannin Oenothein B in Plant Materials by HPLC‐DAD‐MS: Method Development and Validation
Author(s) -
Granica Sebastian,
Bazylko Agnieszka,
Kiss Anna K.
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2358
Subject(s) - chemistry , chromatography , extraction (chemistry) , high performance liquid chromatography , ellagitannin , herb , medicinal herbs , polyphenol , organic chemistry , traditional medicine , antioxidant , medicine
Oenothein B (OeB) is a dimeric macrocyclic ellagitannin occurring mainly in the genus Oenothera . In the literature no validated HPLC method for quantification of this compound has been reported. Objective To develop and validate an efficient HPLC method for quantification of OeB in plant materials. Methodology Extraction conditions of OeB from plant material were optimised. Then the first validated HPLC method for quantification was developed. Results We have shown that ultrasonic‐assisted extraction at 40 °C in 15 min using water are the most optimal conditions for OeB extraction. We have quantified OeB in several plant materials belonging to Oenotheraceae family. The lowest amount of OeB was present in Circaea lutetiana herb (27.64 ± 0.26 mg/g) and the highest was quantified in Epilobium hirsutum aerial parts (72.91 ± 1.38 mg/g). Conclusion The first HPLC method for quantification of OeB in plant materials was developed and validated. We also for the first time optimised the extraction conditions for OeB. Copyright © 2012 John Wiley & Sons, Ltd.