Premium
Simultaneous Extraction and Separation of Flavonols and Flavones from Chamaecyparis obtusa by Multi‐phase Extraction using an Ionic Liquid‐modified Microsphere Polymer
Author(s) -
Tian Minglei,
Bi Wentao,
Row Kyung Ho
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2357
Subject(s) - chemistry , amentoflavone , myricetin , quercitrin , sorbent , chromatography , extraction (chemistry) , flavones , organic chemistry , rutin , flavonoid , kaempferol , adsorption , antioxidant
Flavonols and flavones, especially quercitrin, myricetin and amentoflavone, are the main anti‐bacterial and anti‐cancer compounds in Chamaecyparis obtuse . Multi‐phase extraction is a new method that can extract and separate target compounds simultaneously. Objective An amino ionic liquid immobilised microsphere polymer was used as a multi‐phase extraction sorbent to extract and separate quercitrin, myricetin and amentoflavone from Chamaecyparis obtusa . Methodology The sorbent and Chamaecyparis obtusa powder were packed into a single cartridge. Using a fixed volume of methanol with five repetitions, the target compounds were extracted from the powder to the sorbent. The sorbent was then washed with n ‐hexane to remove any interfering species and the target compounds were eluted sequentially using water, methanol and methanol containing 1% acetic acid (vol.). Results Under the optimised conditions, 0.45 mg/g of quercitrin, 0.18 mg/g of myricetin and 0.12 mg/g of amentoflavone from 2.0 g of powder were obtained by multi‐phase extraction using 0.3 g of sorbent. Conclusion The method described has a low deviation error, requires a small amount of solvent and is highly selective and reproducible. Copyright © 2012 John Wiley & Sons, Ltd.