Premium
Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix ( Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology
Author(s) -
Wu Yanfang,
Wang Xinsheng,
Fan Enguo
Publication year - 2012
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2349
Subject(s) - puerarin , isoflavones , chemistry , extraction (chemistry) , chromatography , pueraria , response surface methodology , radix (gastropod) , box–behnken design , botany , biochemistry , medicine , alternative medicine , pathology , biology
Isoflavones, particularly puerarin, are a group of important components from Puerariae Lobatae Radix (PLR) which show diverse pharmacological activities, therefore giving rise to the development of various extraction methods. Ultrasound‐assisted extraction (UAE) has been explored for isoflavone extraction from PLR as it provides higher extraction efficiency compared with traditional methods. Objective To optimise the UAE conditions for puerarin and total isoflavone extraction from PLR using response surface methodology (RSM). Methodology Samples were prepared by use of ultrasound‐assisted extraction, and then subjected to HPLC analysis. Box–Behnken Design (BBD), a widely used form of RSM, was used for the optimisation of the UAE process. Results The effects of ethanol concentration, extraction time and the solvent‐to‐material ratio on the yields of puerarin and total isoflavones were investigated. The Box–Behnken experimental results demonstrate that optimal extraction was obtained with an ethanol concentration of 71.35%, an extraction time of 49.08 min and a solvent‐to‐material ratio of 21.72 for puerarin, and an ethanol concentration of 80.00%, an extraction time of 55.00 min and a solvent‐to‐material ratio of 12.81 for total isoflavones. The yields of puerarin and total isoflavones were 41 ± 0.63 mg/g and 128 ± 0.82 mg/g, respectively, under the optimised extraction conditions, which are in agreement with the values predicted by the RSM. Conclusion The RSM allows for optimising the extraction parameters such that maximum extraction of puerarin and total isoflavones was achieved experimentally. Copyright © 2012 John Wiley & Sons, Ltd.