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Quantitative and Transformation Product Analysis of Major Active Physalins from Physalis Alkekengi Var. Franchetii (Chinese Lantern) Using Ultraperformance Liquid Chromatography with Electrospray Ionisation Tandem Mass Spectrometry and Time‐of‐flight Mass Spectrometry
Author(s) -
Zheng Yunliang,
Chen Yong,
Ren Yiping,
Luan Lianjun,
Wu Yongjiang
Publication year - 2011
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1363
Subject(s) - chemistry , chromatography , physalis , electrospray ionization , calyx , tandem mass spectrometry , mass spectrometry , high performance liquid chromatography , electrospray , liquid chromatography–mass spectrometry , analytical chemistry (journal) , traditional medicine , botany , medicine , biology
Chinese lantern is the calyx or calyx‐with‐fruit of the plant Physalis alkekengi .var. franchetii (Solanaceae) , and is potential material for the food and pharmaceutical industries. Physalins are the most active and representative secondary metabolites of Chinese lantern. A separation and quantification method based on UPLC‐ESI‐MS/MS was developed for the quantitative analysis of five active physalins. The transformation products were also detected and identified for the first time. Objective To establish a LC‐MS/MS method to quantify five physalins in Chinese lantern for the purpose of quality control, and to identify the transformation products of 4,7‐didehydrophysalin B. Methodology The separation was carried out on an Acquity UPLC BEH Shield RP C 18 ‐column with water and acetonitrile as the mobile phase under gradient conditions. ESI‐MS/MS was used as the detector to quantify the five physalins. The transformation products of 4,7‐didehydroneophysalin B were detected by UPLC–PDA‐ESI‐MS/MS and identified through comparing their HRMS and MS 2 ion fragmentations with corresponding references. Results All the compounds showed good linearity ( R 2 > 0.998). The recoveries, measured at three concentration levels, varied from 98.8 to 101.4% with RSDs < 4.5%. The total contents of the five physalins in Chinese lantern varied significantly. Three transformation products of 4,7‐didehydroneophysalin B were detected and tentatively identified. Conclusion The present study developed a highly effective analytical method for the quality control of Chinese lantern, and it could provide comprehensive information for quality evaluation and new drug development of Chinese lantern. Copyright © 2011 John Wiley & Sons, Ltd.