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Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High‐speed Counter‐current Chromatography
Author(s) -
Jia Jing,
Chen Tao,
Wang Ping,
Chen Guichen,
You Jinmao,
Liu Yongjun,
Li Yulin
Publication year - 2011
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1362
Subject(s) - chemistry , countercurrent chromatography , chromatography , high performance liquid chromatography , traditional medicine , medicine
Xanthones, the primary constituents of Swertia mussotii , are known to possess a variety of biological activities, including anti‐depressant, anti‐leukaemic, anti‐tumour, anti‐tubercular, choleretic, diuretic, anti‐microbial, anti‐fungal, anti‐inflammatory, anti‐viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high‐speed counter‐current chromatography (HSCCC). Methodology A solvent system composed of n ‐hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL/min, a rotation speed of 800 rpm and a temperature of 25 °C. Results Using the described method, 8 mg of methylswertianin, 21 mg of swerchirin and 11 mg of decussatin with purities of over 98% could be isolated from a 150 mg crude sample. They were identified by 1 H‐NMR and 13 C‐NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright © 2011 John Wiley & Sons, Ltd.