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Two‐dimensional Hydrophilic Interaction/Reversed‐phase Liquid Chromatography for the Preparative Separation of Polar and Non‐polar Taxanes
Author(s) -
Rezadoost Hassan,
Ghassempour Alireza
Publication year - 2011
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1338
Subject(s) - chemistry , taxane , chromatography , polar , countercurrent chromatography , hydrophilic interaction chromatography , high performance liquid chromatography , taxus , reversed phase chromatography , paclitaxel , column chromatography , chemical polarity , fraction (chemistry) , organic chemistry , botany , molecule , medicine , physics , surgery , cancer , astronomy , chemotherapy , breast cancer , biology
Paclitaxel (Taxol ® ) is one of the best‐known compounds of natural origin that has been used in the pharmaceutical industry. It is derived from taxanes extracted from different parts of yew trees (Taxaceae), cell cultures and taxane‐producing endophytic fungi. Previous work reported a semi‐preparative HPLC for the extraction of Paclitaxel (a non‐polar taxane) from Taxus baccata L. and cell culture. In other work, 10‐deacetylbaccatin III (a polar taxane) was separated from this medium at a preparative scale. Objective To introduce a novel on‐line two‐dimensional heart‐cut liquid chromatography using hydrophilic interaction/reversed‐phase chromatography for preparative separation of polar and non‐polar taxanes. Methodology To achieve this goal the system was developed from analytical to a larger preparative scale. In the first dimension of a semi‐preparative column, taxanes were separated from polar compounds using a CN column, and in the second dimension the fraction containing taxanes was transferred to a C 18 ‐column. Results Using this method polar and non‐polar taxanes could be separated at analytical and semi‐preparative scales. Conclusion Combination of hydrophilic and reversed‐phase chromatography in two‐dimensional liquid chromatography (2D‐LC) systems is a useful method for the separation of compounds with a large polarity range such as taxanes. Copyright © 2011 John Wiley & Sons, Ltd.