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Preparative Isolation, Fast Centrifugal Partition Chromatography Purification and Biological Activity of Cajaflavanone from Derris ferruginea Stems
Author(s) -
Morel Sylvie,
Landreau Anne,
Nguyen Van Hung,
Derbré Séverine,
Grellier Philippe,
Le Pape Patrice,
Pagniez Fabrice,
Litaudon Marc,
Richomme Pascal
Publication year - 2011
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1336
Subject(s) - chemistry , chromatography , fractionation , column chromatography , biological activity , in vitro , biochemistry
ABSTRACT Introduction The Derris genus is known to contain flavonoid derivatives, including prenylated flavanones and isoflavonoids such as rotenoids, which are generally associated with significant biological activity. Objective To develop an efficient preparative isolation procedure for bioactive cajaflavanone. Methodology Fast centrifugal partition chromatography (FCPC) was optimised to purify cajaflavanone from Derris ferruginea stems in a single step as compared to fractionation from the cyclohexane extract by successive conventional solid–liquid chromatography procedures. The purification yield, purity, time and solvent consumption per procedure are described. The anti‐fungal, anti‐bacterial, anti‐leishmanial, anti‐plasmodial, anti‐oxidant activities and the inhibition of advanced glycation end‐products (AGEs) by cajaflavanone accumulation are described. Results FCPC enabled cajaflavanone purification in a single separation step, yielding sufficient quantities to perform in vitro biological screening. Interestingly, cajaflavanone had an inhibitory effect on the formation of AGEs, without displaying any in vitro anti‐oxidant activity. Conclusion A simple and efficient procedure, in comparison with other preparative methods, for bioactive cajaflavone purification has been developed using FCPC. Copyright © 2011 John Wiley & Sons, Ltd.