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Rapid Simultaneous Determination of Major Isoflavones of Pueraria lobata and Discriminative Analysis of its Geographical Origins by Principal Component Analysis
Author(s) -
Zhao Chenxi,
Chan Hoiyan,
Yuan Dalin,
Liang Yizeng,
Lau Tsuiyan,
Chau FooTim
Publication year - 2011
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1308
Subject(s) - chemistry , pueraria , isoflavones , principal component analysis , lobata , discriminative model , chromatography , traditional medicine , statistics , biochemistry , artificial intelligence , medicine , alternative medicine , pathology , mathematics , computer science
Isoflavones are main bioactive components of Pueraria lobata (Willd.) Ohwi. Puerarin has been used as the marker compound in herb quality evaluation in the Chinese Pharmacopoiea. However, it is also important to include the other isoflavones present in the herb, such as daidzin, daidzein, genistin and genistein, in the evaluation as they also contribute to the overall bioactivity of the herb. Objective To develop a rapid and reliable method for simultaneous quantitation of isoflavones for P. lobata herb quality evluation. Methodology The chromatographic separation was performed on an Agilent rapid resolution liquid chromatographic system through gradient elution. The developed method for the quantification of puerarin, daidzin, daidzein, genistin and genistein was fully validated. When it was applied to analyse the extracts of P. lobata , baseline separation was obtained within 10 min. Results The amounts of puerarin, daidzin, daidzein and genistin varied greatly among the samples although their chromatographic fingerprints were similar to each other. The 19 samples studied were classified into three clusters (I–III) by principal component analysis based on the amounts of puerarin, daidzin, daidzein and genistin. Conclusion The classification result can be related to herbal origins, but the classification outcome from the chromatographic fingerprinting similarity approach did not provide any geographical origin information. This shows that bioactive constituents can reflect the intrinsic quality of P. lobata more accurately. Copyright © 2011 John Wiley & Sons, Ltd.