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Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detection
Author(s) -
Lu Binan,
Liu Yuetao,
Yin Lianhong,
Wang Xiaona,
Peng Jinyong
Publication year - 2009
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.1138
Subject(s) - chemistry , chromatography , phosphoric acid , triethylamine , high performance liquid chromatography , repeatability , gradient elution , analyte , methanol , elution , detection limit , analytical chemistry (journal) , organic chemistry
Traditional Chinese medicine plays a very important role in the healthcare system of China and thus the quality control of medicinal herb products is of paramount concern. Objective To establish a simple and effective high‐performance liquid chromatography (HPLC) method to evaluate the quality of Daochi pill. Methodology Two HPLC methods were developed for the determination of 16 marker components in Daochi pills. In method A, the analytes were separated on a Lichrosorb C 18 column using a gradient elution of methanol and 1% aqueous phosphoric acid (pH 2.9, adjusted by triethylamine). In method B, the separation was achieved on an Agilent Eclipse Plus C 18 column using a gradient elution of methanol and 3% aqueous phosphoric acid (pH 2.0, adjusted by triethylamine) in a gradient elution mode. Results Methods were linear over the range 0.27–500 µg/mL ( r 2 ≥ 0.9995). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at three concentrations were higher than 95% with RSD ≤ 4% for the targets. Conclusion The established methods are simple and can be used as tools for quality evaluation and control of this natural product. Copyright © 2009 John Wiley & Sons, Ltd.